Alpha&ESM hFolds

A database for the comparison of structural models predicted by ESMFold and AlphaFold2 for human proteins.
Now updated with Quality Assessment and Functional characterization of the models.
Part of the Bioinformatics Sweeties collection.

Protein information Download JSON
Protein name Galactose-1-phosphate uridylyltransferase
Gene name GALT
UniProt accession P07902
Sequence length 379
Protein source SwissProt (reviewed)
Signal/Transit peptide Absent
Highest-coverage PDB Chain 6gqd_A
PDB Chain coverage 0.91
Experimental method (Resolution) X-ray diffraction (1.52 Å)

Sequence alignment (from structure superimposition) Download FASTA

Model Confidence: Very high (pLDDT ≥ 90) High (90 > pLDDT ≥ 70) Low (70 > pLDDT ≥ 50) Very low (pLDDT < 50)

Structure superimposition
ESMFold
AlphaFold2
Structure alignment scores
TM-score 0.9
RMSD 1.99 Å
GDT 0.84
TM-score pLDDT≥50 0.95
TM-score pLDDT≥70 0.98
TM-score pLDDT≥90 0.99
Sequence coverage
ESMFold start - end 20 - 379
AlphaFold2 start - end 21 - 379
Alignment length 362
Match positions 347
Mismatch positions 10
Gap positions (Gap opens) 22 (4)
Self-assessment ESMFold AlphaFold2
Mean pLDDT 85.32 91.67
External validation ESMFold AlphaFold2
DeepAccNet mean pLDDT 82.22 98.66
QMEANDisCo mean pLDDT 76.6 84.6
QATEN mean pLDDT 69.29 70.11

AlphaFold is better than ESMFold according to 3/3 external QA tools

 Name Type Start End TM-score ESMFold
pLDDT 		AlphaFold2
pLDDT
GalP_UDP_transf Domain 23 196 0.95 85.0 93.18
GalP_UDP_tr_C Domain 203 368 0.94 86.69 97.35
Position Wild type Mutated type Description
1 M R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs771702963
1 M T Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs771702963
1 M V Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033639
9 Q H GALAC1 (from: UniProt); dbSNP:rs111033637
23 T A GALAC1 (from: UniProt); dbSNP:rs111033635
28 D H GALAC1 (from: UniProt); dbSNP:rs111033636
28 D Y GALAC1 (from: UniProt); dbSNP:rs111033636
29 H R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132341442
31 H N Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033643
32 I N GALAC1; mild (from: UniProt); dbSNP:rs111033644
33 R H GALAC1 (from: UniProt); dbSNP:rs111033829
33 R P GALAC1 (from: UniProt)
34 Y H
34 Y N GALAC1; affects protein stability (from: UniProt); dbSNP:rs111033836
38 Q P GALAC1 (from: UniProt); dbSNP:rs111033646
40 E K ; dbSNP:rs886042060
44 V L GALAC1 (from: UniProt); dbSNP:rs111033647
44 V M GALAC1; reduced enzyme activity (from: UniProt); dbSNP:rs111033647
45 S L GALAC1 (from: UniProt); dbSNP:rs111033652
45 S P Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132341560
48 R C Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs886042088
48 R S Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs886042088
51 R L GALAC1 (from: UniProt); dbSNP:rs111033648
51 R Q GALAC1 (from: UniProt); dbSNP:rs111033648
52 P R ; dbSNP:rs2132341621
53 W R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1131691837
55 G C GALAC1 (from: UniProt); dbSNP:rs111033654
67 R C GALAC1 (from: UniProt); dbSNP:rs111033658
67 R H Variant assessed as Somatic; MODERATE impact. (from: NCI-TCGA); dbSNP:rs758430398
68 H P Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs193922247
68 H R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs193922247
74 L P GALAC1; reduced enzyme activity (from: UniProt); dbSNP:rs111033663
74 L R
81 A P Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033665
81 A T GALAC1 (from: UniProt); dbSNP:rs111033665
83 G V GALAC1 (from: UniProt)
89 Y D Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033666
89 Y H GALAC1 (from: UniProt); dbSNP:rs111033666
97 N D Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1564100957
97 N I Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033669
97 N K ; dbSNP:rs398123180
97 N S GALAC1 (from: UniProt); dbSNP:rs111033669
98 D H GALT-related disorder (from: ClinVar); dbSNP:rs111033670
98 D N GALAC1 (from: UniProt); dbSNP:rs111033670
100 P R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1057524572
103 Q R GALAC1 (from: UniProt); dbSNP:rs367543252
112 S R GALAC1 (from: UniProt); dbSNP:rs367543254
113 D N GALAC1 (from: UniProt); dbSNP:rs111033677
114 H L GALAC1 (from: UniProt); dbSNP:rs111033678
117 F S GALAC1 (from: UniProt); dbSNP:rs111033679
118 Q H GALAC1 (from: UniProt); dbSNP:rs111033673
123 R G GALAC1 (from: UniProt); dbSNP:rs111033674
123 R Q GALAC1 (from: UniProt); dbSNP:rs111033675
125 V A GALAC1 (from: UniProt); dbSNP:rs111033680
127 K E GALAC1 (from: UniProt); dbSNP:rs111033682
129 M L Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs193922248
129 M T GALAC1 (from: UniProt); dbSNP:rs111033683
130 C Y GALAC1 (from: UniProt); dbSNP:rs367543255
132 H Q GALAC1; affects protein stability (from: UniProt); dbSNP:rs367543256
132 H Y GALAC1 (from: UniProt); dbSNP:rs111033688
135 S A Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132343082
135 S L GALT-related disorder (from: ClinVar); dbSNP:rs111033690
135 S W GALAC1 (from: UniProt); dbSNP:rs111033690
138 T M Variant assessed as Somatic; MODERATE impact. (from: NCI-TCGA); dbSNP:rs111033686
138 T R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033686
139 L P GALAC1 (from: UniProt); dbSNP:rs111033687
142 M K GALAC1; 4% of normal activity (from: UniProt); dbSNP:rs111033695
142 M T GALAC1 (from: UniProt); dbSNP:rs111033695
142 M V GALAC1 (from: UniProt); dbSNP:rs111033692
143 S L GALAC1 (from: UniProt); dbSNP:rs111033697
143 S W Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033697
147 I V
148 R G GALAC1 (from: UniProt); dbSNP:rs111033693
148 R Q GALAC1 (from: UniProt); dbSNP:rs111033694
148 R W Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033693
150 V L GALAC1 (from: UniProt); dbSNP:rs111033699
151 V A GALAC1; approximately 3% of normal activity (from: UniProt); dbSNP:rs111033701
154 W G GALAC1 (from: UniProt); dbSNP:rs111033702
154 W R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033702
156 S L Variant assessed as Somatic; MODERATE impact. (from: NCI-TCGA); dbSNP:rs766896883
157 V I Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1173928130
166 P A GALAC1 (from: UniProt); dbSNP:rs367543257
167 W C
167 W G Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033708
167 W R GALAC1 (from: UniProt); dbSNP:rs111033708
168 V L GALAC1; loss of activity (from: UniProt); dbSNP:rs367543258
168 V M Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs367543258
169 Q H Variant assessed as Somatic; MODERATE impact. (from: NCI-TCGA)
169 Q K Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033709
170 I T GALAC1; loss of activity (from: UniProt); dbSNP:rs111033839
171 F S GALAC1; reduced enzyme activity (from: UniProt); dbSNP:rs111033715
172 E K
172 E Q
175 G D GALAC1; strongly reduces galactose uridylyltransferase activity (from: UniProt); dbSNP:rs111033718
177 M T Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132343692
179 G D GALAC1 (from: UniProt); dbSNP:rs111033720
181 S A GALAC1 (from: UniProt); dbSNP:rs111033828
181 S F GALAC1 (from: UniProt); dbSNP:rs367543259
183 P T GALAC1 (from: UniProt); dbSNP:rs111033721
184 H D Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033716
184 H Q GALAC1 (from: UniProt); dbSNP:rs111033717
184 H Y Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033716
185 P H GALAC1; loss of activity (from: UniProt); dbSNP:rs111033722
185 P L GALAC1 (from: UniProt); dbSNP:rs111033722
185 P S GALAC1 (from: UniProt); dbSNP:rs111033826
186 H N Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033725
186 H Q Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs886042097
186 H Y Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033725
188 Q K ; dbSNP:rs398123182
188 Q R GALAC1; most common mutation; 10% of normal galactose uridylyltransferase activity; impairs protein folding (from: UniProt); dbSNP:rs75391579
190 W R GALT-related disorder (from: ClinVar); dbSNP:rs398123184
191 A D Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs794727838
191 A V Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs794727838
192 S G GALAC1 (from: UniProt); dbSNP:rs111033830
192 S N GALAC1 (from: UniProt); dbSNP:rs111033734
194 F L GALAC1 (from: UniProt); dbSNP:rs111033726
195 L P GALT-related disorder (from: ClinVar); dbSNP:rs111033728
195 L R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033728
196 P A
196 P L Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs886042066
197 D G GALT-related disorder (from: ClinVar); dbSNP:rs1554709359
198 I M GALAC1 (from: UniProt); dbSNP:rs111033729
198 I T GALAC1 (from: UniProt); dbSNP:rs1483461355
199 A T GALAC1 (from: UniProt); dbSNP:rs111033730
201 R C Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033739
201 R H GALAC1 (from: UniProt); dbSNP:rs111033735
201 R P
202 E K Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132344159
203 E G Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132344172
203 E K GALAC1 (from: UniProt); dbSNP:rs111033736
204 R * Variant assessed as Somatic; HIGH impact. (from: NCI-TCGA); dbSNP:rs111033737
204 R L
204 R P GALAC1 (from: UniProt); dbSNP:rs111033740
204 R Q Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033740
206 Q R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1587239309
207 Q E
209 Y C GALAC1 (from: UniProt); dbSNP:rs111033744
209 Y D Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132344222
209 Y S GALAC1 (from: UniProt); dbSNP:rs111033744
212 Q H GALAC1 (from: UniProt)
217 L P GALAC1 (from: UniProt); dbSNP:rs111033741
220 E K GALAC1; 3-fold decrease in activity (from: UniProt); dbSNP:rs111033747
223 R C Premature ovarian failure (from: ClinVar); dbSNP:rs111033750
223 R H Variant assessed as Somatic; MODERATE impact. (from: NCI-TCGA); dbSNP:rs553769637
223 R L
223 R S GALAC1; 3-fold decrease in activity (from: UniProt); dbSNP:rs111033750
226 L P GALAC1 (from: UniProt); dbSNP:rs111033752
227 L P GALAC1; results in no detectable protein in the soluble fraction (from: UniProt); dbSNP:rs111033846
229 K N GALAC1 (from: UniProt); dbSNP:rs111033753
231 R C GALAC1 (from: UniProt); dbSNP:rs111033749
231 R G Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033749
231 R H GALAC1; 15% of normal activity (from: UniProt); dbSNP:rs111033754
244 P S GALAC1 (from: UniProt)
249 W R GALAC1 (from: UniProt); dbSNP:rs111033757
250 P T ; dbSNP:rs111033759
251 Y C GALAC1 (from: UniProt); dbSNP:rs111033755
251 Y S Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs886043390
251 Y S GALAC1 (from: UniProt); dbSNP:rs111033755
252 Q H GALAC1 (from: UniProt); dbSNP:rs111033769
258 R C GALAC1 (from: UniProt); dbSNP:rs368166217
259 R Q GALAC1; loss of activity (from: UniProt); dbSNP:rs886042070
259 R W Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs786204763
262 R P GALAC1 (from: UniProt); dbSNP:rs111033763
265 P A GALAC1 (from: UniProt); dbSNP:rs111033764
267 L R GALAC1 (from: UniProt)
267 L V GALAC1 (from: UniProt)
271 E D GALAC1 (from: UniProt); dbSNP:rs1262475195
271 E G Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033765
272 R C Variant assessed as Somatic; MODERATE impact. (from: NCI-TCGA); dbSNP:rs111033766
272 R G GALAC1 (from: UniProt); dbSNP:rs111033766
272 R H Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033831
272 R L
275 L V Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs796051967
278 I N GALAC1; 18-fold decrease in activity (from: UniProt); dbSNP:rs111033778
279 M R Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033779
279 M T
279 M V
282 L V GALAC1 (from: UniProt); dbSNP:rs111033772
285 K N GALAC1; severe; impairs protein folding; nearly abolishes enzyme activity (from: UniProt); dbSNP:rs111033773
285 K Q
285 K R GALAC1 (from: UniProt); dbSNP:rs367543263
289 L F GALAC1; 3-fold decrease in activity (from: UniProt); dbSNP:rs111033774
289 L R GALAC1 (from: UniProt); dbSNP:rs111033775
291 E K GALAC1 (from: UniProt); dbSNP:rs111033780
291 E V GALAC1; 2-fold decrease in activity (from: UniProt); dbSNP:rs111033841
294 F Y GALAC1 (from: UniProt); dbSNP:rs111033781
295 P T Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033783
298 M I Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1821187550
298 M V Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1821187430
299 G S Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1587240180
300 W C
304 P S Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs2132346162
308 E K GALAC1 (from: UniProt); dbSNP:rs111033784
314 N D GALT-related disorder (from: ClinVar); dbSNP:rs2070074
317 Q H GALAC1 (from: UniProt); dbSNP:rs111033787
317 Q R GALAC1 (from: UniProt); dbSNP:rs111033786
319 H Q GALAC1 (from: UniProt); dbSNP:rs111033792
320 A T GALAC1 (from: UniProt); dbSNP:rs111033795
321 H Y GALT-related disorder (from: ClinVar); dbSNP:rs367543266
323 Y C Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs367543267
323 Y D GALAC1 (from: UniProt); dbSNP:rs111033796
323 Y H GALAC1 (from: UniProt); dbSNP:rs111033796
324 P S GALAC1 (from: UniProt); dbSNP:rs111033798
325 P L GALAC1 (from: UniProt); dbSNP:rs111033794
327 L P GALAC1; results in no detectable protein in the soluble fraction (from: UniProt); dbSNP:rs111033832
328 R C Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs144993986
328 R H GALAC1 (from: UniProt); dbSNP:rs111033802
329 S F GALAC1 (from: UniProt); dbSNP:rs111033803
329 S P ; dbSNP:rs398123188
330 A V GALAC1; mild (from: UniProt); dbSNP:rs111033804
333 R G GALAC1 (from: UniProt); dbSNP:rs111033800
333 R L GALAC1 (from: UniProt); dbSNP:rs111033808
333 R Q GALAC1 (from: UniProt); dbSNP:rs111033808
333 R W Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033800
334 K R GALAC1 (from: UniProt); dbSNP:rs111033809
336 M L GALAC1 (from: UniProt); dbSNP:rs111033810
336 M V Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs111033810
340 E K Variant assessed as Somatic; MODERATE impact. (from: NCI-TCGA); dbSNP:rs111033806
342 L I GALAC1 (from: UniProt); dbSNP:rs111033812
344 Q K GALAC1 (from: UniProt); dbSNP:rs111033814
345 A D GALAC1 (from: UniProt); dbSNP:rs111033815
350 T A GALAC1; mild (from: UniProt); dbSNP:rs111033817
350 T I Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs775317639
350 T N Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs775317639
350 T S
352 E G Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs1821203474
352 E Q
353 Q E
363 E K Deficiency of UDPglucose-hexose-1-phosphate uridylyltransferase (from: ClinVar); dbSNP:rs367543273

Sequence alignment (from structure superimposition) Download FASTA

Model Confidence: Very high (pLDDT ≥ 90) High (90 > pLDDT ≥ 70) Low (70 > pLDDT ≥ 50) Very low (pLDDT < 50)

Structure superimposition
ESMFold
PDB (6gqd_A)
Structure alignment scores
TM-score 0.92
RMSD 2.3 Å
TM-score pLDDT≥50 0.95
TM-score pLDDT≥70 0.96
TM-score pLDDT≥90 0.98
Sequence coverage
ESMFold start - end 18 - 370
PDB start - end 1 - 344
Alignment length 355
Match positions 318
Mismatch positions 24
ESMFold gap positions 2
PDB gap positions 37
Gap opens 9

Sequence alignment (from structure superimposition) Download FASTA

Model Confidence: Very high (pLDDT ≥ 90) High (90 > pLDDT ≥ 70) Low (70 > pLDDT ≥ 50) Very low (pLDDT < 50)

Structure superimposition
AlphaFold2
PDB (6gqd_A)
Structure alignment scores
TM-score 0.97
RMSD 1.28 Å
TM-score pLDDT≥50 0.97
TM-score pLDDT≥70 0.97
TM-score pLDDT≥90 0.99
Sequence coverage
AlphaFold2 start - end 24 - 370
PDB start - end 4 - 344
Alignment length 347
Match positions 330
Mismatch positions 11
AlphaFold2 gap positions 3
PDB gap positions 38
Gap opens 3