Alpha&ESM hFolds

A database for the comparison of structural models predicted by ESMFold and AlphaFold2 for human proteins.
Now updated with Quality Assessment and Functional characterization of the models.
Part of the Bioinformatics Sweeties collection.

Information

External resources

The list of proteins is obtained from querying UniProt (Release 2025_04). The lists of pathogenic SRVs are also retrieved from UniProt.
AlphaFold models are downloaded from AlphaFold Protein Structure Database (V6 models).
ESMFold models are generated in house using ESMFold.
Experimental structures are retrieved from the Protein Data Bank (accessed November 2025).
Pfam entries are annotated using the PfamScan tool against the Pfam database (Release 37.0).
External validation is performed by running in house DeepAccNet, QMEANDisCo, and QATEN.

Changelog

XX/XX/2025: The list of proteins have been updated to be aligned with the latest releases of UniProt (Release 2025_04) and AlphaFold DB (V6). The database now includes 48,815 human proteins.
09/06/2025: The database now includes an external validation of the models, and a functional annotation of the models with the mapping of Pfam entries and pathogenic variations. The advanced search has also been updated to be more dynamic and to include filters based on the new information available.
21/03/2024: First release